Open AccessAntibiofilm activity of mushroom extracts against Staphylococcus aureus
Author(s) -
Anna Čuvalová,
I. Strapáč,
Lívia Handrová,
V. Kmeť
Publication year - 2018
Publication title -
annales universitatis paedagogicae cracoviensis. studia naturae
Language(s) - English
Resource type - Journals
eISSN - 2545-0999
pISSN - 2543-8832
DOI - 10.24917/25438832.3supp.2
Subject(s) - mushroom , biofilm , staphylococcus aureus , microbiology and biotechnology , biology , food science , bacteria , genetics
Mushrooms are a renowned source of products with an array of bioactivities, from antibacterial to antiviral, cytotoxic, antifeeding, antifungal or antioxidant and might be a valuable resource in the search of new bioactive extracts to inhibit biofilm production. We demonstrate the effect of five mushroom water extracts Macrolepiota procera, Pleurotus ostreatus, Auricularia auricula-judae, Armillaria mellea and Laetiporus sulphurous on biofilm formation of four Staphylococcus aureus strains isolated from ixodid ticks (Acari) and ewe´s milk. The PCR was used for detection of virulence genes (hla, isdA, B, bbp, sirB, fnbpA, sdrE, agr II). The ability of biofilm formation and anti-biofilm activity of mushrooms extracts was assessed in a quantitative crystal violet assay. The biofilm formation of S. aureus strains was significantly reduced by all mushrooms extracts (p < 0.001). We showed that more significant anti-biofilm effect of the extracts was of Staphylococcus aureus isolated from ixodid ticks in comparison to Staphylococcus aureus isolated from ewe´s milk. In the present study, A. mellea, P. ostreatus, L. sulphurous, A. auricula-judae and M. procera extracts inhibited biofilm formation by 70.87%, 67.00%, 64.14%, 62.77% and 47.71%, respectively. The results suggest that compounds in mushrooms extracts might be useful to control and handle detrimental infections caused by animal and human pathogens.