Open AccessEFEKTIVITAS PEMBERIAN EKSTRAK BIJI KETUMBAR TERHADAP GAMBARAN SEL BUSA ATEROSKLEROSIS AORTA ABDOMINALIS TIKUS HIPERKOLESTEROLEMIA DIABETES
Author(s) -
Kusvandita Giopratiwi,
Maria Selvester Thadeus,
Retno Yulianti
Publication year - 2020
Publication title -
jurnal muara sains, teknologi, kedokteran, dan ilmu kesehatan
Language(s) - English
Resource type - Journals
eISSN - 2579-6410
pISSN - 2579-6402
DOI - 10.24912/jmstkik.v4i2.8250
Subject(s) - alloxan , traditional medicine , medicine , abdominal aorta , aorta , significant difference , negative control , diabetes mellitus , endocrinology
Coriander Seed Extract (CSE) is thought to have the potential to repair foam cell formation process which is part of atherosclerotic lesion. The aim of this study is to identify the effects of coriander seed extract on histopathological features of atherosclerotic foam cells abdominal aorta in rats hypercholesterolemia diabetes model. The study was conducted for 63 days. 30 Wistar outbred rats were grouped into five groups, each group consist of 6 rats. Negative control group (standar feed), positive control group (high fat diet and alloxan + glibenclamide 0.045 mg), treatment group 1 (high fat diet and alloxan + CSE 300 mg/kgBB/day), treatment group 2 (high fat diet and alloxan + CSE 500 mg/kgBB/day), treatment group 3 (high fat diet and alloxan + CSE 700 mg/kgBB/day). After the treatment were given to each group, the next procedure were dissection, extraction of the aorta abdominal organ, and histopathological examination of foam cell. Data were analyzed with One Way ANOVA test and Post Hoc Bonferoni test show there are significant difference (P value = 0.049) between positive control group and treatment group 3. In conclusion, CSE 700 mg/kgBB dose can reduce foam cell formation. Keywords: atherosclerosis; coriander seed extract; foam cell; hypercholesterolemia. ABSTRAK Ekstrak biji ketumbar (EBJ) diduga memiliki potensi untuk memperbaiki proses pembentukan sel busa lesi aterosklerosis. Penelitian ini bertujuan untuk mengetahui efektivitas pemberian ekstrak biji ketumbar terhadap gambaran histopatologi sel busa aterosklerosis aorta abdominalis tikus model hiperkolesterolemia diabetes. Penelitian dilakukan selama 63 hari. Sebanyak 30 ekor tikus Galur Wistar outbred dibagi ke dalam 5 kelompok, masing-masing kelompok terdiri dari 6 ekor. Kelompok kontrol negatif (pakan standar), kelompok kontrol positif (pakan tinggi lemak dan aloksan + glibenklamid 0.045 mg), kelompok perlakuan 1 (pakan tinggi lemak dan aloksan + EBJ 300 mg/kgBB/hari), kelompok perlakuan 2 (pakan tinggi lemak dan aloksan + EBJ 500 mg/kgBB/hari), dan kelompok perlakuan 3 (pakan tinggi lemak dan aloksan + EBJ 700 mg/kgBB/hari). Setelah perlakuan, dilakukan pembedahan dan pengambilan organ aorta abdominalis lalu dibuat preparat untuk menilai gambaran sel busa. Analisis statistika dengan Uji One Way ANOVA dan uji Post Hoc Bonferoni menunjukkan ada perbedaan signifikan (P Value=0.049) antara kelompok kontrol positif dengan kelompok perlakuan 3. Kesimpulannya, EBJ dosis 700 mg/kgBB/hari memiliki efektivitas untuk menurunkan pembentukan sel busa dalam proses aterosklerosis.