Open AccessMolecular detection of mecA gene from Methicillin Resistant Coagulase Negative Staphylococci
Author(s) -
Arun Thangave,
R. Chandrasekaran,
Omprakash Sahu,
Saravanan Ponnappan,
Gezahegn Abawa,
Abenezer Tadele
Publication year - 2017
Publication title -
journal of microbiology and biotechnology research
Language(s) - English
Resource type - Journals
ISSN - 2231-3168
DOI - 10.24896/jmbr.2017723
Subject(s) - coagulase , sccmec , microbiology and biotechnology , agarose gel electrophoresis , biology , staphylococcus , agarose , genomic dna , cons , dna , agar , dna extraction , polymerase chain reaction , gene , staphylococcus aureus , methicillin resistant staphylococcus aureus , bacteria , genetics , computer science , programming language
In the present research, a total of 27 Wound swab samples were collected for the isolation of Methicillin Resistant-Coagulase Negative Staphylococci. Out of these, 3.7% of the samples have been shown the presence of Methicillin Resistant-CoagulaseNegative Staphylococci (MR-CoNS). The preliminary coagulase activity has been identified by Coagulase slide and tube method. The HiCrome MeReSa agar medium has been used for the preliminary identification of Methicillin resistance. During the isolation, the other strains of Coagulase Negative Staphylococci (29.6%), Methicillin Sensitive Staphylococcus aureus (44.4%) have been also isolated. 6 samples (22.2%) were sterile. The genomic DNA was isolated from Methicillin resistant Coagulase Negative Staphylococciand other species of Staphylococcithrough Modified DNA extraction method. The isolated DNA was amplified through the thermal cycler using suitable forwardand reverse oligonucleotide primers, for the amplification of 527bp of mecA gene. The amplified DNA product has been identified by agarose gel electrophoresis using suitable 100bp ladder marker.