Open AccessThe method of heterotopic rat heart transplantation for investigation the cardioprotective efficacy of cardioplegic solutions
Author(s) -
С. М. Минасян,
Я. И. Полещенко,
П. Ю. Шубина,
Е. С. Процак,
Д. А. Дружининский,
М. М. Галагудза,
И. С. Усков,
Ю. В. Дмитриев
Publication year - 2019
Publication title -
regionarnoe krovoobraŝenie i mikrocirkulâciâ
Language(s) - English
Resource type - Journals
eISSN - 2712-9756
pISSN - 1682-6655
DOI - 10.24884/1682-6655-2019-18-3-59-67
Subject(s) - cardioprotection , medicine , heart transplantation , transplantation , heart transplants , preservative , anesthesia , surgery , cardiology , chemistry , ischemia , food science
This article describes in detail the modied method of heterotopic heart transplantation in rats. The methods of animal anesthesia, surgical features of the removal of a donor heart and its implantation to a recipient rat are described in detail. The advantages and disadvantages of donor heart preservation methods are described, as well as methods for evaluating a donor heart preparation, the quality of preservation and the effectiveness of cardioprotection. Experimental approaches to the study of the effectiveness of cardioplegic and preservative solutions using this technique are proposed. The data of our own pilot study on a comparative analysis of the effectiveness of cardioprotection using preservative solutions using this experimental model are presented.