Open AccessMyocardial progenitor cells as an option for cell therapy for congenital heart disease
Author(s) -
Anna Malashicheva,
A. A. Sabirova,
И. А. Козырев,
А. С. Головкин,
A. A. Hudiakov,
Anna Kostareva
Publication year - 2015
Publication title -
regionarnoe krovoobraŝenie i mikrocirkulâciâ
Language(s) - English
Resource type - Journals
eISSN - 2712-9756
pISSN - 1682-6655
DOI - 10.24884/1682-6655-2015-14-3-52-58
Subject(s) - progenitor cell , medicine , heart disease , clonogenic assay , cell therapy , regenerative medicine , population , stem cell , cell type , pathogenesis , pathology , immunology , cell , biology , microbiology and biotechnology , genetics , environmental health
Myocardial progenitor cells represent a perspective source for cell therapy of cardiovascular disorders and are intensively studied. However, mainly the cells from adult patients are studied whereas the cells derived from children with congenital heart diseases remained poorly investigated. The aim of the present work was to obtain progenitor cells from intraoperatively obtained cardiac tissue from infants and children undergoing repair of congenital cardiac defects. Following isolation, the cells gave rise to a clonogenic, highly proliferative spindle-shaped cell population. The cells expressed markers of cardiogenic origin and were shown to differentiate towards cardiogenic lineage. This resident myocardial progenitor cells obtained from infant myocardial tissue demonstrate similar characteristics to previously described cells derived from adult myocardial tissue. This study confirms the possibility of obtaining a pool of progenitor cells from tiny tissue fragments and opens a new perspective of using these cells in regenerative medicine and further research of congenital heart disease pathogenesis.