Open AccessDNA POLYMORPHISM AND GENETIC DIVERSITY OF MANGO (Mangifera sp.) GERMPLASM IN TROPICAL ISLAND
Author(s) -
Putu Suwardike,
I Nyoman,
Rindang Dwiyani,
Eniek Kriswiyanti
Publication year - 2020
Publication title -
international journal of biosciences and biotechnology/international journal of bioscience and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2655-9994
pISSN - 2303-3371
DOI - 10.24843/ijbb.2019.v07.i01.p05
Subject(s) - germplasm , genetic diversity , biology , mangifera , microsatellite , genotype , genetic relationship , genetic similarity , forensic science , horticulture , veterinary medicine , allele , botany , genetics , microbiology and biotechnology , gene , medicine , population , environmental health
Creation of new superior varieties of mango through the empowerment of local genetic resources requires information about the potential properties of mango germplasm, including DNA polymorphism and its genetic diversity. This research aimed to obtain basic data on DNA polymorphism, genetic relationship, genetic similarity level, and molecular accession of Bali’s unique local mango. Sampling was conducted in four regencies in the Province of Bali. DNA preparation, PCR, and microsatellite analysis were carried out at the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Gadjah Mada University. It found 44accessions of mangoes in Bali. The test results with 10 pairs of SSR markers showed that all primers produced polymorphic loci. There were 825 amplified DNA bands. Primer of AY31 produced the highest number of loci, which were 14 loci, while AY21 produced the fewest loci, i.e., 3 loci. Forty-four mango accessions showed a genetic similarity coefficient of 0.27 to 0.97. At a coefficient of 0.27, accessions were divided into 2 major groups: group A and group B. Group A consisted of two accessions, namely, KRA-005 and BDG-006 (Mangifera foetida Lour.), which had a similar coefficient of 0.657. Group B was divided into twosmaller groups, namely, groups B1 and B2, at a similarity coefficient of 0.342. Group B1 consisted of 39 accessions, while group B2 consisted of 3 accessions. Accessions Madu Anggur, Gading, Sambuk Mengwi, Kakul and Pakel Sulangai were identified as having unique alleles.