Open AccessDifficult to Treat Proteeae strains in high risk Romanian hospital departments
Author(s) -
Corina Musuroi,
Monica Licker,
Maria Rus,
Edward Șeclăman,
Delia Muntean,
Silvana Vulpie,
Luminița Bădițoiu
Publication year - 2021
Publication title -
revista română de medicină de laborator
Language(s) - English
Resource type - Journals
eISSN - 2284-5623
pISSN - 1841-6624
DOI - 10.2478/rrlm-2021-0003
Subject(s) - proteus mirabilis , medicine , antibiotics , intensive care , case fatality rate , antibiotic resistance , emergency medicine , intensive care medicine , microbiology and biotechnology , pseudomonas aeruginosa , biology , epidemiology , bacteria , genetics
Introduction : Resistance to first-line antibiotics of the Proteeae strains within the difficult-to-treat (DTR) phenotype is a cause of limitation of therapeutic options. The study aimed to characterize these strains, to identify the factors that influence their acquisition and the predictive factors for the patient’s evolution. Material and methods : Between July 2017 and January 2019, 400 of Proteeae strains were isolated from samples of patients admitted to intensive care units (ICUs) and surgical wards of a university hospital in Romania. The identification and testing of antibiotic sensitivity was performed using the Vitek 2 Compact system. The DTR phenotype was defined as the resistance (or intermediate resistance) to all categories of β-lactams, carbapenems and fluoroquinolones. Results : Out of 400 Proteeae strains, 21% were of the DTR type, most of them from the species Providencia stuartii and Proteus mirabilis, identified predominantly on the ICUs. The excess fatality in the DTR subsample compared to the non-DTR subsample was 16.37%. The multivariate analysis identified as independent risk factors: the number of antibiotics administered, the number of days of urinary catheterization, the presence of tracheostomy, nasogastric nutrition, respectively belonging to the species P. stuartii. The probabilities of survival were reduced by the presence of the central venous catheter (CVC), tracheostomy, by the increase of the number of hospitalization days respectively of the number of antibiotics administered. Conclusion : The DTR phenotype in the case of Proteeae strains has been associated especially with the species P. stuartii, with invasive exogenous factors and with an increased fatality.