Open AccessOccurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018
Author(s) -
Wojciech Kozdruń,
Natalia Styś-Fijoł,
Hanna Czekaj,
Karolina Piekarska,
Jowita Samanta Niczyporuk,
Agnieszka Stolarek
Publication year - 2020
Publication title -
journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.469
H-Index - 25
eISSN - 2450-8608
pISSN - 2450-7393
DOI - 10.2478/jvetres-2020-0079
Subject(s) - flock , marek's disease , disease , biology , virology , virus , spleen , broiler , veterinary medicine , medicine , pathology , immunology , zoology
Marek’s disease (MD) is a tumourous disease caused by Marek’s disease virus (MDV) and most commonly described in poultry. The aim of the study was to determine the occurrence of Marek’s disease virus infections in Poland and analyse clinical cases in the years 2015–2018. Material and Methods The birds for diagnostic examination originated from 71 poultry flocks of various types of production. Birds were subjected to anatomopathological examination post mortem, during which liver and spleen sections and other pathologically changed internal organs were taken. These sections were homogenised with generally accepted methods, then total DNA was isolated and amplified with a real-time PCR. A pair of primers complementary to the MDV genome region encoding the meq gene were used. Results MDV infection was found predominantly in broiler chicken flocks (69.01%), and also in layer breeder (9.85%) and commercial layer flocks (7.04% each). Conclusion The results of research conducted in the years 2015–2018 clearly indicate that the problem of MDV infections is still current.