Open AccessMolecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland
Author(s) -
Monika KrajewskaWędzina,
Monika Kozińska,
Łukasz Radulski,
Marek Lipiec,
Ewa AugustynowiczKopeć,
Marcin Weiner,
Krzysztof Szulowski
Publication year - 2020
Publication title -
journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.469
H-Index - 25
eISSN - 2450-8608
pISSN - 2450-7393
DOI - 10.2478/jvetres-2020-0003
Subject(s) - outbreak , mycobacterium bovis , herd , zoonosis , genotyping , veterinary medicine , tuberculosis , transmission (telecommunications) , biology , bovine tuberculosis , molecular epidemiology , mycobacterium tuberculosis , virology , genotype , medicine , genetics , electrical engineering , engineering , pathology , gene
Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis / caprae strains and determination of the epidemiological relationship of various bTB outbreaks. Material and Methods The material for microbiological tests came from 119 cattle ( Bos taurus ) from nine herds located in five provinces, neighbouring the Masovian Province. Results Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.