Open AccessEvaluation of long-term antibody response and cross-serotype reaction in ducks immunised with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN
Author(s) -
May Phonvisay,
Jai-Wei Lee,
Jhong-Jie Liou,
Hsian-Yu Wang,
Chun-Yen Chu
Publication year - 2019
Publication title -
journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.469
H-Index - 25
eISSN - 2450-8608
pISSN - 2450-7393
DOI - 10.2478/jvetres-2019-0066
Subject(s) - cpg oligodeoxynucleotide , adjuvant , antibody , serotype , biology , vaccination , virology , recombinant dna , antigen , titer , immunology , immune system , gene expression , gene , dna methylation , biochemistry
Riemerella anatipestifer (RA) infections can lead to high mortality in ducklings. Inactivated vaccines against RA are commercially available, but they fail to provide cross-protection against various serotypes. We have previously demonstrated that a subunit vaccine containing recombinant outer membrane protein A (rOmpA) antigen of serotype 2 formulated with CpG oligodeoxynucleotides (ODN) as the adjuvant was able to stimulate both humoral and cellular immunities. Material and Methods In the present study, thirty healthy 7-day-old Pekin ducks were randomly assigned to three equal treatment groups: rOmpA-vaccinated, rOmpA + CpG-vaccinated, and control. Vaccine was injected intramuscularly and a booster dose of the same vaccine was given two weeks after primary immunisation. The long-term antibody response and cross-serotype reaction of this vaccine were evaluated in ducks. Results Compared to ducks immunised with rOmpA alone, ducks immunised with rOmpA + CpG ODN had significantly (p < 0.05) increased serum antibody titre from two weeks until nine months after primary immunisation. In addition, expression of cytokines including interferon (IFN)-α, IFN-γ, interleukin (IL)-6, and IL-12 was significantly (p < 0.05) enhanced in PBMC of ducks immunised with rOmpA + CpG ODN two weeks after primary immunisation. Antibodies from ducks immunised with the rOmpA + CpG ODN vaccine could also detect RA serotypes 1 and 6 in Western blot analysis. Conclusion Combination of rOmpA and CpG ODN could be a feasible strategy for developing a subunit RA vaccine with long term and broader-ranging protection.