Open AccessDevelopment of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses
Author(s) -
Yali Liu,
Yaozhong Ding,
Jing Dai,
Bing Ma,
Jijun He,
Weimin Ma,
Jing Lv,
Xiaoyuan Ma,
Yunwen Ou,
Jun Wang,
Yongsheng Liu,
Huiyun Chang,
Yonglu Wang,
Zhang Qiang,
Xiangtao Liu,
Yongguang Zhang,
Jie Zhang
Publication year - 2018
Publication title -
journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.469
H-Index - 25
eISSN - 2450-8608
pISSN - 2450-7393
DOI - 10.2478/jvetres-2018-0064
Subject(s) - virology , serotype , biology , foot and mouth disease virus , foot and mouth disease , amplicon , virus , primer (cosmetics) , multiplex polymerase chain reaction , polymerase chain reaction , aphthovirus , gene , genetics , chemistry , organic chemistry
The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information. Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay. Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results. Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.