Open AccessCytotoxic roles of apigenin and kaempferol on staurosporine-treated mesenchymal stem cells in an in vitro culture
Author(s) -
Magdalena Kosiedowska,
Arkadiusz Burczak,
Julia Morys,
Paulina Borkowska,
Jan Kowalski
Publication year - 2021
Publication title -
herba polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.169
H-Index - 4
eISSN - 2449-8343
pISSN - 0018-0599
DOI - 10.2478/hepo-2021-0022
Subject(s) - apigenin , kaempferol , staurosporine , chemistry , mesenchymal stem cell , cytotoxic t cell , in vitro , pharmacology , apoptosis , flavonoid , traditional medicine , biology , biochemistry , kinase , medicine , protein kinase c , microbiology and biotechnology , antioxidant
Summary Introduction Flavonoids are widely distributed in the wild. They constitute a large group of compounds that have a beneficial effect on the human body. Apigenin and kaempferol, which belong to the flavone subgroup, have, inter alia , an antitumor effect. The influence of these compounds on the survival of stem cells in a toxic environment has not yet been studied. Objective The aim of the study was to evaluate the effect of selected concentrations of apigenin and kaempferol on the survival of mesenchymal stem cells (MSC) in the presence of a cell-death inducer – staurosporine. Methods Mesenchymal stem cells that were obtained from the Wharton’s jelly of umbilical cords were used for the research. In the first stage, the MSC were treated with apigenin at concentrations of 1.2, 12.5, 25, 50 and 100 µ M/ml and kaempferol at concentrations of 1.2, 12.5, 25, 50 and 100 µ M/ml. In the next stage, the effect of increased concentrations of 0.1, 0.5 and 1 µ M/ml of staurosporine on MSC was examined. The key stage of the experiment was investigating the interaction between the selected concentrations of apigenin (12.5 and 50 µ M/ml) and kaempferol (12.5 and µ M/ml) on MSC in the presence of staurosporine at a concentration of 1 µ M/ml, which had the highest toxicity. Results Both apigenin and kaempferol significantly increased the cytotoxic features of staurosporine on the MSC culture.