Open AccessIn Vitro Inhibitory Activity of Cell-Free Supernatants of Lactobacillus Spp. and Bacillus Spp. Against Fusarium graminearum
Author(s) -
M. Harčárová,
E. Čonková,
P. Naď,
P. Váczi,
Martina Proškovcová
Publication year - 2021
Publication title -
folia veterinaria
Language(s) - English
Resource type - Journals
eISSN - 2453-7837
pISSN - 0015-5748
DOI - 10.2478/fv-2021-0022
Subject(s) - lactobacillus fermentum , bacillus licheniformis , bacillus subtilis , lactobacillus plantarum , fusarium , lactobacillus , lactobacillus reuteri , microbiology and biotechnology , biology , minimum inhibitory concentration , bacillus (shape) , food science , bacteria , botany , fermentation , antimicrobial , lactic acid , genetics
In this study, the antifungal activity of cell-free supernatant (CFS) of Lactobacillus spp. ( Lactobacillus plantarum CCM 1904; L81, Lactobacillus fermentum ; 2I3, Lactobacilus reuteri ; 2/6, L26;) and Bacillus spp. ( Bacillus subtilis CCM 2794, Bacillus licheniformis CCM 2206) against two strains of Fusarium graminearum CCM F-683 and Fusarium graminearum CCM 8244 were investigated in vitro . All tested CFS of Lactobacillus spp. were able to inhibit the growth of both strains of Fusarium graminearum . The highest inhibitory effect (IE) (56.5 %) against F. graminearum CCM F-683 was observed for CFS Lactobacillus fermentum (2I3) at the minimum inhibitory concentration (MIC) (2.25 ± 0.56 mg.ml –1 ). CFS of Lactobacillus reuteri (2/6) showed the best IE (40.0 %) against F. graminearum CCM 8244 (2/6) at the MIC 1.25 mg.ml –1 . However, no inhibitory effect of Bacillus subtilis and Bacillus licheniformis CFS against both strains of F. graminearum were observed, even at the highest tested concentration of 5.0 mg.ml –1 .