Open AccessAnalysis of Sister Chromatid Exchanges and Proliferation of Human Peripheral Blood Lymphocytes Exposed to Epoxiconazole
Author(s) -
Romana Michálková,
Katarı́na Šiviková,
Martina Galdíková
Publication year - 2018
Publication title -
folia veterinaria
Language(s) - English
Resource type - Journals
eISSN - 2453-7837
pISSN - 0015-5748
DOI - 10.2478/fv-2018-0026
Subject(s) - sister chromatid exchange , pi , peripheral blood , sister chromatids , chemistry , cytotoxic t cell , medicine , endocrinology , in vitro , cytotoxicity , positive control , negative control , biology , biochemistry , traditional medicine , chromosome , gene
The potential genotoxic/cytotoxic effect of epoxiconazole was evaluated by means of sister chromatid exchanges (SCE) following the 24 and 48 h in vitro exposure of human peripheral blood lymphocytes to epoxiconazole at concentrations of: 5, 10, 25, 50 and 100 μg. ml –1 . Dimethyl sulphoxide (DMSO), used as an epoxiconazole solvent, was used as a negative control and mitomycine (MMC) as a positive control. After the 24-hour exposure, we failed to observe a significant increase in SCE frequencies in comparison with the negative control, however, the concentrations of 10—100 μg.ml –1 caused a significant decrease in the proliferation index (PI; P < 0.001). Also, the 48-hour exposure produced no significant alterations in the SCE frequencies in comparison with the control. At epoxiconazole concentrations ranging from 10 to 50 μg.ml –1 we recorded a moderate to strong, dose-dependent inhibition of PI (P < 0.05; P < 0.01; P < 0.001), while at the highest dose (100 μg.ml –1 ) the reduction in PI compared to the control was less pronounced (P < 0.05). The reduction in PI at the concentration range of 10—100 μg.ml –1 depended on the number of cells in the M 1 , M 2 and M 3 phases of the cell cycle per total number of 100 evaluated metaphases. Our results indicated a significant cytotoxic or cytostatic effect on human peripheral blood lymphocytes.