Open AccessArtificial cell microcapsules containing live bacterial cells and activated charcoal for managing renal failure creatinine: preparation and in-vitro analysis
Author(s) -
Trisna Lim,
Wei Ouyang,
Christopher Martoni,
Nasri Balit,
Satya Prakash
Publication year - 2019
Publication title -
the eurobiotech journal
Language(s) - English
Resource type - Journals
ISSN - 2564-615X
DOI - 10.2478/ebtj-2019-0023
Subject(s) - activated charcoal , creatinine , lactobacillus acidophilus , in vitro , charcoal , chemistry , metabolite , chromatography , urea , activated carbon , blood urea nitrogen , adsorption , bacteria , pharmacology , biochemistry , probiotic , biology , organic chemistry , genetics
Activated charcoal was microencapsulated with Lactobacillus acidophilus 314 previously adapted for urea uptake. The creatinine removal capacity of this combination microcapsule was evaluated in-vitro in media simulating the small intestine. Results show that microcapsules containing both activated charcoal and L. acidophilus 314 demonstrated potential for decreasing creatinine. Interestingly, when co-encapsulating both activated charcoal and L. acidophilus 314 a smaller decrease in creatinine was observed than when encapsulating them separately. However, co-encapsulated microcapsules were more stable in various parts of the gastrointestinal system and survived longer in storage. These results suggest the feasibility of using microcapsules containing activated charcoal and probiotic bacteria as oral adjuvants for creatinine removal and provides a theoretical model for the use of these microcapsules to remove any unwanted metabolite.