Open AccessTannins, novel inhibitors of the volume regulation and the volume-sensitive anion channel
Author(s) -
Nargiza A. Tsiferova,
Ozoda J. Khamidova,
Anvar Amonov,
M. B. Rakhimova,
S. I. Rustamova,
R. Sh. Kurbannazaova,
Petr G. Merzlyak,
Nodira Abdulladjanova,
Ravshan Z. Sabirov
Publication year - 2019
Publication title -
european pharmaceutical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.151
H-Index - 7
ISSN - 2453-6725
DOI - 10.2478/afpuc-2019-0016
Subject(s) - chemistry , tannin , volume (thermodynamics) , proanthocyanidin , biochemistry , reversion , botany , food science , biology , polyphenol , physics , quantum mechanics , phenotype , gene , antioxidant
The volume-sensitive outwardly rectifying anion channel (VSOR) is a key component of volume regulation system critical for cell survival in non-isosmotic conditions. The aim of the present study was to test the effects of four tannin extracts with defined compositions on cell volume regulation and VSOR. Preparation I (98% of hydrolysable tannins isolated from leaves of sumac Rhus typhina L. ) and Preparation II (100% of hydrolysable tannins isolated from leaves of broadleaf plantain Plantago major L ) completely and irreversibly abolished swelling-activated VSOR currents in HCT116 cells. Both preparations profoundly suppressed the volume regulation in thymocytes with half-maximal effects of 40.9 μg/ml and 12.3 μg/ml, respectively. The inhibition was more efficient at lower concentrations but reverted at higher doses due to possible non-specific membrane-permeabilizing activity. Preparations III and IV (54,7% and 54.3% of hydrolysable tannins isolated, respectively, from roots and aboveground parts of Fergana spurge Euphorbia ferganensis B.Fedtch ) inhibited VSOR activity in a partially reversible manner and suppressed the volume regulation with substantially higher half-maximal doses of 270 and 278 μg/ml, respectively, with no secondary reversion at higher doses. Hydrolysable tannins represent a novel class of VSOR channel inhibitors with the capacity to suppress the cell volume regulation machinery.