Open AccessEpithelium morphogenesis and oviduct development are regulated by significant increase of expression of genes after long-term in vitro primary culture – a microarray assays
Author(s) -
Katarzyna Stefańska,
Agata Chamier-Gliszczyńska,
Maurycy Jankowski,
Piotr Celichowski,
Magdalena Kulus,
Magdalena Rojewska,
Paweł Antosik,
Dorota Bukowska,
M Bruska,
Michał Nowicki,
Bartosz Kempisty,
Michal Ješeta,
Jana Žáková
Publication year - 2018
Publication title -
medical journal of cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.294
H-Index - 8
ISSN - 2544-3577
DOI - 10.2478/acb-2018-0030
Subject(s) - oviduct , morphogenesis , biology , microbiology and biotechnology , epithelium , microarray analysis techniques , embryo , embryogenesis , gene expression , microarray , gene , genetics , endocrinology
The correct oviductal development and morphogenesis of its epithelium are crucial factors influencing female fertility. Oviduct is involved in maintaining an optimal environment for gametes and preimplantation embryo development; secretory oviductal epithelial cells (OECs) synthesize components of oviductal fluid. Oviductal epithelium also participates in sperm binding and its hyperactivation. For better understanding of the genetic bases that underlay porcine oviductal development, OECs were isolated from porcine oviducts and established long-term primary culture. A microarray approach was utilized to determine the differentially expressed genes during specific time periods. Cells were harvested on day 7, 15 and 30 of in vitro primary culture and their RNA was isolated. Gene expression was analyzed and statistical analysis was performed. 48 differentially expressed genes belonging to “tube morphogenesis”, “tube development”, “morphogenesis of an epithelium”, “morphogenesis of branching structure” and “morphogenesis of branching epithelium” GO BP terms were selected, of which 10 most upregulated include BMP4, ARG1, SLIT2, FGFR1, DAB2, TNC, EPAS1, HHEX, ITGB3 and LOX. The results help to shed light on the porcine oviductal development and its epithelial morphogenesis, and show that after long-term culture the OECs still proliferate and maintain their tube forming properties.