Open AccessRB137 and RB138 antibodies recognize human cathelicidin LL37 by ELISA
Author(s) -
Raffaella Palazzo,
Immacolata Pietraforte,
Carlo Chizzolini,
Roberto Lande,
Loredana Frasca
Publication year - 2020
Publication title -
antibody reports
Language(s) - English
Resource type - Journals
ISSN - 2624-8557
DOI - 10.24450/journals/abrep.2020.e188
Subject(s) - cathelicidin , antibody , recombinant dna , citrullination , antimicrobial peptides , peptide , chemistry , immunology , medicine , biochemistry , citrulline , amino acid , arginine , gene
LL37 is a cationic antimicrobial peptide (AMP), which can undergo post-translational modifications (PTM), such as citrullination and carbamylation. We demonstrate here that recombinant antibodies RB137 and RB138 are specific for native LL37 and do not recognize modified LL37 (citrullinated and carbamylated). They thus represent tools to assess the presence of native, unmodified LL37 in body fluids by ELISA.