Open AccessDO JASMONIC ACID AND ACTIVATED CHARCOAL INCREASE THE in vitro DEVELOPMENT OF ORANGE CARROT (Daucus carota L.) AND PURPLE CARROT (Daucus carota L. ssp. sativus var. atrorubens Alef.)?
Author(s) -
Tuğçe Özsan,
Gülsün Gülsün Elif Vural,
Ahmet Naci Onus
Publication year - 2020
Publication title -
acta scientiarum polonorum. hortorum cultus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.252
H-Index - 20
eISSN - 2545-1405
pISSN - 1644-0692
DOI - 10.24326/asphc.2020.5.12
Subject(s) - daucus carota , jasmonic acid , plantlet , activated charcoal , botany , germination , shoot , biology , horticulture , murashige and skoog medium , cotyledon , chemistry , explant culture , in vitro , biochemistry , organic chemistry , adsorption , gene
Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.