Open AccessEVALUATION OF GENETIC VARIABILITY WITHIN SWEET CHERRY (Prunus avium L.) GENETIC RESOURCES BY MOLECULAR SSR MARKERS
Author(s) -
Josef Patzak,
Alena Henychová,
F. Paprštein,
J. Sedlák
Publication year - 2019
Publication title -
acta scientiarum polonorum. hortorum cultus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.252
H-Index - 20
eISSN - 2545-1405
pISSN - 1644-0692
DOI - 10.24326/asphc.2019.3.15
Subject(s) - biology , prunus , dendrogram , germplasm , loss of heterozygosity , genetic variability , genetic marker , cultivar , genetic diversity , botany , genetics , genotype , gene , allele , population , demography , sociology
Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for fu- ture breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.