Open AccessCell Imaging with Fluorescent Bi-Metallic Nanoparticles
Author(s) -
Natalia Ye. Stasyuk,
Galina Gayda,
Roman Ja. Serkiz,
Mykhailo Gonchar
Publication year - 2015
Publication title -
journal of advances in chemistry
Language(s) - English
Resource type - Journals
ISSN - 2321-807X
DOI - 10.24297/jac.v11i4.6694
Subject(s) - fluorescence , fluorescence microscope , fluorescence lifetime imaging microscopy , microscopy , biomolecule , nanotechnology , materials science , live cell imaging , nanoparticle , biophysics , chemistry , cell , biochemistry , pathology , biology , optics , medicine , physics
Last decades various imaging techniques have been applied in biological and biomedical research, such as magnetic resonance imaging, different types of tomography, fluorescence/bioluminescence, ultrasound, as well as multimodality approaches. Fluorescence imaging, especially in combination with nanoscale materials, is a very prospective tool for experiments in vivo and clinical applications due to its high temporal and spatial resolutions. Fluorescent nanoparticles (NPs), having ability to interact with biomolecules both on the surface of and inside the cells, may revolutionize the cell imaging approaches for diagnostics and therapy. In our investigation we report about new method of cell imaging with fluorescent bi-metallic NPs synthesized by chemical reduction of the relevant ions. As the model of living organism, the cells of yeast Hansenula polymorpha were used. All NPs in minimal concentration (up to 0.05 mM) was proved to be non-toxic for yeast cells. The NPs and NPs-modified cells were characterized with the methods of UV-VIS spectroscopy, scanning electron microscopy, atom force microscopy, transmission electron microscopy and fluorescence microscopy. The bimetallic NPs, possessing the stable fluorescence in solution and inside the cells, allow to observe the phenomenon of NPs transferring from parental to daughter cells through at least three generations followed by releasing from the modified cells. The fluorescent NPs synthesized being small, non-toxic and fluorescent was shown to be perspective tool for cell imaging.