Open AccessGLUT 4 DI JARINGAN ADIPOSA (GLUT 4 in Adipose Tissue)
Author(s) -
Dewi Ratna Sari,
Rimbun Rimbun,
Tri Hartini Yuliawati,
Joni Susanto,
Anak Agung Ngurah Gunawan,
Harjanto Jm
Publication year - 2018
Publication title -
indonesian journal of clinical pathology and medical laboratory
Language(s) - English
Resource type - Journals
ISSN - 2477-4685
DOI - 10.24293/ijcpml.v21i1.1263
Subject(s) - adipose tissue , medicine , endocrinology , insulin resistance , diabetes mellitus , immunohistochemistry , cholecalciferol , obesity , vitamin d and neurology , streptozotocin , analysis of variance
The decreasing of glucose transporter 4 (GLUT 4) in adipose tissue of diabetic and obesity patients are associated withhyperinsulinaemia and insulin resistance. The adipose tissue can be used as therapeutic targets in the treatment of Diabetes Mellitus(DM). Visceral adipose tissue has different morphology and functional with subcutaneous adipose tissue and Vitamin D has been knownto have contributed in DM. The aim of this study is to know the role of cholecalciferol on the expression of GLUT 4 in subcutaneous andvisceral adiposity of diabetic rats by elucidated in those tissues. The subjects of the study consisted of nineteen male diabetic rats of Wistarstrain, which were divided into control group (K) and three (3) treatment groups (X1, X2 and X3). In order to induce the condition ofDM, the animals were fed with high fat diet for three (3) weeks and administered a single intraperitoneal injection of streptozotocin (35mg/kgBW) at the end of the second week. Cholecalciferol were administered with doses of 6.25 μg/kgBW in X1, 12.5 μg/kgBW in X2and 25 μg/kgBW in X3 per oral everyday within 14 days. The subcutaneous and visceral adipose tissues of the subjects were processedinto histological slides with immunohistochemistry staining. The data were analyzed by one way ANOVA test and paired t-test (α= 0.05,significance p 0.05). Based on thisstudy it can be concluded that cholecalciferol could increase the expression of GLUT 4 in the subcutaneous adiposity, but not in visceraladiposity of the diabetic rats.