Open AccessSingle-cell RNA sequencing reveals atlas of dairy goat testis cells
Author(s) -
Xiuwei Yu,
Tongtong Li,
Xiaomin Du,
Qiaoyan Shen,
Meng-Fei Zhang,
Yudong Wei,
Donghui Yang,
Wenjing Xu,
Wenbo Chen,
Chunling Bai,
Xueling Li,
Guangpeng Li,
Na Li,
Sha Peng,
Mingzhi Liao,
Na Li
Publication year - 2021
Publication title -
zoological research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 19
ISSN - 2095-8137
DOI - 10.24272/j.issn.2095-8137.2020.373
Subject(s) - rna , atlas (anatomy) , biology , computational biology , microbiology and biotechnology , genetics , anatomy , gene
Single-cell RNA sequencing (scRNA-seq) is useful for exploring cell heterogeneity. For large animals, however, little is known regarding spermatogonial stem cell (SSC) self-renewal regulation, especially in dairy goats. In this study, we described a high-resolution scRNA-seq atlas derived from a dairy goat. We identified six somatic cell and five spermatogenic cell subtypes. During spermatogenesis, genes with significantly changed expression were mainly enriched in the Notch, TGF-β, and Hippo signaling pathways as well as the signaling pathway involved in the regulation of stem cell pluripotency. We detected and screened specific candidate marker genes ( TKTL1 and AES ) for spermatogonia. Our study provides new insights into goat spermatogenesis and the development of testicular somatic cells.