Open AccessChromosome-level genome assembly of the Chinese longsnout catfish <i>Leiocassis longirostris</i>
Author(s) -
Wei He,
Jian Zhou,
Zhe Li,
Tingsen Jing,
Chunhua Li,
Y J Yang,
Mengbin Xiang,
Chaowei Zhou,
Guangjun Lv,
Hongyan Xu,
Hongyu Luo,
Hua Ye
Publication year - 2021
Publication title -
zoological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 19
ISSN - 2095-8137
DOI - 10.24272/j.issn.2095-8137.2020.327
Subject(s) - contig , biology , genome , catfish , genetics , phylogenetic tree , chromosome , sequence assembly , karyotype , genome size , reference genome , evolutionary biology , gene , fish <actinopterygii> , fishery , transcriptome , gene expression
The Chinese longsnout catfish ( Leiocassis longirostris Günther) is one of the most economically important freshwater fish in China. As wild populations have declined sharply in recent years, it is also a valuable model for research on sexual dimorphism, comparative biology, and conservation. However, the current lack of high-quality chromosome-level genome information for the species hinders the advancement of comparative genomic analysis and evolutionary studies. Therefore, we constructed the first high-quality chromosome-level reference genome for L. longirostris . The total genome was 703.19 Mb, with 389 contigs and contig N50 length of 4.29 Mb. Using high-throughput chromosome conformation capture (Hi-C) data, the genome sequences (685.53 Mb) were scaffolded into 26 chromosomes ranging from 17.36 to 43.97 Mb, resulting in a chromosomal anchoring rate for the genome of 97.44%. In total, 23 708 protein-coding genes were identified in the genome. Phylogenetic analysis indicated that L. longirostris and its closest related species P. fulvidraco diverged approximately 26.6 million years ago. This high-quality reference genome of L. longirostris should pave the way for future genomic comparisons and evolutionary research.