Open AccessHistopathological and Ultrastructural Changes in the Liver and Gills of the Killifish Aphanius Dispar (Cyprinodontidae) Exposed to Aflatoxin B1
Author(s) -
Horiya H. Al-Azri,
Taher Ba-Omar,
Abdulkadir E. Elshafie,
Michael J. Barry
Publication year - 2015
Publication title -
maǧallaẗ ǧāmiʿaẗ al-sulṭān qābūs li-l-ʿulūm/sultan qaboos university journal for science
Language(s) - English
Resource type - Journals
eISSN - 2414-536X
pISSN - 2308-3921
DOI - 10.24200/squjs.vol20iss1pp1-10
Subject(s) - gill , ultrastructure , biology , dispar , killifish , aflatoxin , zoology , toxicity , in vivo , anatomy , fish <actinopterygii> , fishery , microbiology and biotechnology , medicine , food science , entamoeba histolytica
Aflatoxin B1 (AFB1) is a mycotoxin which can cause serious toxicity to animals and humans. The aim of this study was to investigate the effects of AFB1 in Aphanius dispar fish and measure residues in tissues after in vivo exposure. Aphanius dispar were fed diets containing 50, 100, 150 and 200 µg AFB1/kg for 10, 20 and 30 days. At the end of the experiment, the liver and gills were dissected out and processed for light and electron microscopy. During the experiment, no external changes or unusual behavior were observed in the fish. Histopathological and ultrastructural changes in liver appeared under all four treatments: 50, 100, 150 and 200 µg AFB1/kg. Gill tissues were affected at high doses of 100,150 and 200 µg AFB1/kg. Accumulation of AFB1 residues in liver and gill tissues was found to be related to a dose and duration of exposure.