Open AccessEffect of Different Concentrations of Growth Regulators on Gardenia jasminoides cv. Veitchii Micropropagation by Tissue Culture Technique
Author(s) -
G. Abdullah,
Abdullatif A. Al-Khateeb,
M. Serage
Publication year - 2003
Publication title -
journal of agricultural and marine sciences
Language(s) - English
Resource type - Journals
eISSN - 2410-1079
pISSN - 2410-1060
DOI - 10.24200/jams.vol8iss1pp35-40
Subject(s) - micropropagation , cutting , shoot , sucrose , gardenia jasminoides , horticulture , gardenia , tissue culture , agar , botany , biology , murashige and skoog medium , food science , in vitro , medicine , biochemistry , genetics , alternative medicine , pathology , traditional chinese medicine , bacteria
Micropropagation techniques were set up for Gardenia jasminoides c.v. veitchi. Many plantlets were obtained by culturing shoot cuttings in MS nutrient media, 30 g/L Sucrose, 7 g/L Agar Agar, and different concentrations of BAP and IAA. The best concentration was 1mg /L BAP with 0.5 mg/L IAA. This concentration gave the best sprout growth suitable for rooting in primary and secondary culture by reculturing the stuck cutting every 6 weeks and for many times. We also obtained a high rooting percentage up to 98 % of natural rooting in rooting media different from propagation media by reducing mineral salt concentration to half, Sucrose to 20gm/L, and 2gm/L active charcoal, and 1mg/L IAA. Plantlets were transferred to greenhous and subjected for hardening. This technique gave 22 plantlets from one cutting in one year.