Open AccessEstablishment and In Vitro Propagation of a Putative Variant of Periwinkle
Author(s) -
A. S. AI-Wasel
Publication year - 2000
Publication title -
journal of agricultural and marine sciences
Language(s) - English
Resource type - Journals
eISSN - 2410-1079
pISSN - 2410-1060
DOI - 10.24200/jams.vol5iss1pp15-18
Subject(s) - shoot , explant culture , biology , murashige and skoog medium , botany , catharanthus roseus , micropropagation , elongation , auxin , butyric acid , horticulture , in vitro , food science , materials science , biochemistry , ultimate tensile strength , gene , metallurgy
Shoot multiplication of a putative variant of Catharanthus roseus (L.) G. Don, was achieved in vitro using shoot tips and nodal segments as explants. The addition of growth regulators to establishment medium stimulated bud breaking and shoot elongation. The maximum shoot multiplication (15.1 shoots/microshoot) and the longest shoots (7.0 cm) occurred on Murashige and Skoog medium (MS) containing 1.0 mg L-1 of N6-Benzyladenine (BA) and a- Naphthalene acetic acid (NAA). All microshoots formed roots and normal root morphology occurred on half strength MS salt supplied with 0.5 mg L-1 NAA or Indole-B-Butyric acid (IBA). Rooted microshoots (95 %) were successfully transferred to soil.