Open AccessAntibacterial Activities of 5-Nitro-2-uryl and 5-Nitro-2-Imidazolyl Derivatives of 1,3,4-Thiadiazole
Author(s) -
Mohammad Hassan Moshafi,
Ali Peymani,
Alireza Foroumadi,
Mohammad Reza Zabihi,
Farzad Doostishoar
Publication year - 2019
Publication title -
internal medicine and medical investigation journal
Language(s) - English
Resource type - Journals
ISSN - 2474-7750
DOI - 10.24200/imminv.v4i2.213
Subject(s) - minimum inhibitory concentration , staphylococcus epidermidis , chemistry , antimicrobial , antibacterial activity , bacillus subtilis , nitro , nitrofuran , klebsiella pneumonia , minimum bactericidal concentration , bacteria , serial dilution , gram positive bacteria , escherichia coli , staphylococcus aureus , organic chemistry , biology , biochemistry , medicine , genetics , alkyl , alternative medicine , pathology , gene
Introduction: Nitrofurans and nitroimidazoles are broad-spectrum antimicrobial agents, which affect the microbial DNA. The aim of the present study was to evaluate the new derivatives of these two groups of antimicrobials against certain Gram-positive and Gram-negative bacterial strains. Materials and Methods: Seven new derivatives of nitrofurans and nitroimidazoles were synthesized, and 6.4 mg of each derivative was dissolved in dimethyl sulfoxide. Then, 8 serial dilutions (0.5, 1, 2, 4, 8, 16, 32, and 64 μg/ml) of each derivative was prepared using Muller-Hinton broth, and the minimum inhibitory concentration for each derivative was measured and compared to ciprofloxacin (standard). Results: All the derivatives had no antibacterial effects against Gram-negative bacteria (minimum inhibitory concentration > 64 μg/ml); only 2-(5-nitro-2-furyl)-5-(n-pentylsulfunyl)-1,3,4-thiadiazole exhibited mild antibacterial effects against Klebsiella pneumonia (minimum inhibitory concentration of 16-32 μg/ml). The antibacterial effects of the derivatives against Gram-positive bacteria also showed variations from complete inhibition of the growth of Staphylococcus epidermidis and Bacillus subtilis (minimum inhibitory concentration < 0.5 μg/ml) by 2-(5-nitro-2-furyl)-5-(n-buthylthio)-1,3,4-thiadiazole to no inhibition of S. epidermidis and streptococcus pyogenes. Conclusion: These compounds have weak antibacterial effects; only two derivatives showed antibacterial effects similar to that of the positive control.