Open AccessSulfamethizole functionalized graphene oxide for in-vitro separation and determination lead in blood serum of battery manufactories workers by syringe filter-dispersive- micro solid phase extraction
Author(s) -
Abhijit De,
Seyed Mojtaba Mostafavi
Publication year - 2020
Publication title -
analytical methods of environmental chemistry journal
Language(s) - English
Resource type - Journals
eISSN - 2645-5552
pISSN - 2645-5382
DOI - 10.24200/amecj.v3.i04.124
Subject(s) - adsorption , solid phase extraction , chromatography , extraction (chemistry) , nuclear chemistry , chemistry , elution , materials science , oxide , metallurgy , organic chemistry
The toxic effect of lead (Pb) causes to anemia and iron deficiency in human body. So, the lead determination in blood/serum samples is very important. In this study, a novel adsorbent based on sulfamethizole functionalized on nanographene oxide (C3H10N4O2S2-NGO; SM-NGO) was used for extraction of Pb(II) from human blood, serum and plasma samples in battery manufactories workers by SF-D-µ-SPE. By procedure, 25 mg of SM-NGO mixed with 10 mL of human blood/serum or plasma samples and aspirated by 10 mL of syringe tube. After sonication of samples for 5 min, the Pb ions adsorbed based on sulfur of SM-NGO adsorbent at pH=6 and the solid phase separated by syringe coupled to Millex-FG hydrophobic PTFE membrane (0.2 µm). Then, the lead ions were back-extracted from SM-NGO/PTFE by elution phase with 0.5 mL of nitric acid solution (0.5 M). Finally, the concentrations of Pb(II) ions were determined by AT-FAAS.