Open AccessThe inhibitory effectiveness of ethanol extract of selasih leaves towards Streptococcus sanguis growth
Author(s) -
Ivhatry Rizky Octavia Putri Susilo,
Mieke Hemiawati Satari,
Ame Suciati Setiawan
Publication year - 2015
Publication title -
padjajaran journal of dentistry/padjadjaran journal of dentistry
Language(s) - English
Resource type - Journals
eISSN - 2549-6212
pISSN - 1979-0201
DOI - 10.24198/pjd.vol27no1.26694
Subject(s) - ocimum , basilicum , ethanol , tannin , streptococcus , chemistry , traditional medicine , antibacterial activity , food science , agar diffusion test , minimum inhibitory concentration , agar , bacteria , flavonoid , microbiology and biotechnology , biology , in vitro , biochemistry , antioxidant , medicine , genetics
Selasih is one of tropical and subtropical plant used as traditional medicine. It has antibacterial activity especially toward Gram-positive bacteria because it contains volatile oils, tannin, flavonoid and tertepenoid. Method: The study was performed as an experimental laboratory study used Kirby Bauer agar diffusion applied to eight samples of Streptococcus sanguis which was done by twice repetition for each sample. The concentrations of ethanol extract were 16%, 8%, 4%, 2% and 1%.The result was analysed using ANOVA method for single factor of experimental design. The result of the study showed that there was evidence of the antibacterial effect contained in the ethanol extract of selasih leaves inhibiting the growth of Streptococcus sanguis . The average of inhibitory zone for each concentration in every sample, were: 5,25 mm for 16%, 3,22 mm for 8%, 1,84 mm for 4%, 1,53 mm for 2% and 0,19 mm for 1%. The conclusion of the study indicates that there is an antibacterial effects in ethanol extract of selasih leaves (Ocimum basilicum Linn) which inhibits the growth of Streptococcus sanguis. This antibacterial strength is caused by the active content of selasih leaves extract.Conclusion: The extract of basilicum ethanol (Ocimum basilicum L) effectively inhibits the growth of Streptococcus sanguis.