Open AccessOzonation Pretreatment Evaluation for Xylanase Crude Extract Production from Corncob under Solid-State Fermentation
Author(s) -
Efri Mardawati,
Surya Martha Pratiwi,
Mohamad Djali,
Tita Rialita,
Mohamad Djali,
Yana Cahyana,
Een Sukarminah,
In-In Hanidah,
Imas Siti Setiasih
Publication year - 2017
Publication title -
journal of industrial and information technology in agriculture
Language(s) - English
Resource type - Journals
ISSN - 2548-5059
DOI - 10.24198/jiita.v1i2.14664
Subject(s) - corncob , xylanase , hemicellulose , chemistry , xylan , fermentation , solid state fermentation , autoclave , xylose , food science , substrate (aquarium) , biochemistry , polysaccharide , enzyme , hydrolysis , organic chemistry , biology , raw material , ecology
Xylanases are highly exploited enzyme in industries, including food and chemical industry. Xylanases can be utilized in catalyzing the endohydrolysis of 1,4-β-xylosidic linkages in xylan, lignocellulosic component to produce xylose-monomer. This research aims to optimize xylanase production from alternative substrate, corncob. Corncob contains 41.17% of hemicellulose, polymer of xylan. Xylanases are produced through solid state fermentation by Trichoderma viride. Ratio between substrate and moistening solution was 0.63 g/mL with fermentation temperature 32,8OC. Variables varied include incubation time and pretreatment (using autoclave, ozonation, combination of ozonation and autoclave, also without pretreatment). Xylanase activity was measured by DNS method using 1% of xylan as substrate standard. The result showed that the best incubation time is 36 h with 14403.8707 U/mg protein for specific xylanase activity by using autoclave as pretreatment. Ozonation pretreatment process can increase the enzyme activity of xylanase.