Open AccessIsolation of Estuary Soil Fungi and Screening Antibacterial Activity Against Staphylococcus aureus
Author(s) -
Afrian Rosyadi,
Bawon Triatmoko,
Ari Satia Nugraha
Publication year - 2022
Publication title -
ijpst (indonesian journal pharmaceutival science and technology)/ijpst (indonesian journal of pharmaceutical science and technology)
Language(s) - English
Resource type - Journals
eISSN - 2406-856X
pISSN - 2356-1971
DOI - 10.24198/ijpst.v1i1.28834
Subject(s) - staphylococcus aureus , antibacterial activity , antibiotics , microbiology and biotechnology , penicillin , biology , broth microdilution , bacteria , traditional medicine , medicine , minimum inhibitory concentration , genetics
Infectious disease is one of the causes of death in the world. Antibiotics are often used in the treatment of bacterial infections but are currently experiencing resistance. Based on this, the search for alternative antibiotics from natural sources needs to be done, one of which is from fungi. Fungi are eukaryotic microbes which have been an eminent sources for many currently available antibiotics including penicillin. This study was conducted to determine the potential antibacterial activity of ethyl acetate extract of estuarine fungi isolates against gram-positive, Staphylococcus aureus bacteria. Phytochemical screening of the extract using the TLC method while antibacterial testing was carried through a standard microdilution protocol. Antibacterial activity was reported in percent inhibition. The antibacterial test resulted in seven extracts possessed significant activity, with the highest percent inhibition of the IS-IB-T2 isolate code is 66.5 ± 1.1% and the lowest of the IS-IB-B2 isolate code is 12.2 ± 0.7% at concentration of 100 µg/mL. Majority compounds detected in each extract was terpenoids which suggested the contribution of antibacterial mechanisms. Therefore, estuary soil fungi have the potential to be further explored by antibiotic compounds.Keywords: antibacterial, estuarine fungi, staphylococcus aureus