Open AccessApplication of Whatman FTA Card Method in Oil Palm DNA Extraction and PCR Analysis
Author(s) -
Wan Nurhayati Wan Hanafi,
Nur Syahirah Abu Bakar
Publication year - 2019
Publication title -
scientific research journal/scientific research journal
Language(s) - English
Resource type - Journals
eISSN - 2289-649X
pISSN - 1675-7009
DOI - 10.24191/srj.v16i2.6306
Subject(s) - amplicon , polymerase chain reaction , palm oil , dna extraction , chromatography , agarose gel electrophoresis , dna , biology , palm , agarose , chemistry , food science , biochemistry , gene , physics , quantum mechanics
Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics.