Open AccessStructures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024
Author(s) -
Evelina L. Zdorovenko,
Alexander S. Shashkov,
Alexandra A. Kadykova,
Артем Н. Фахрутдинов,
Е. П. Киселева,
Danuta Tofilevna Plotnikava,
Г. И. Новик,
Yuriy A. Knirel
Publication year - 2017
Publication title -
the eurobiotech journal
Language(s) - English
Resource type - Journals
ISSN - 2564-615X
DOI - 10.24190/issn2564-615x/2017/01.07
Subject(s) - lactococcus lactis , chemistry , polysaccharide , cell wall , dephosphorylation , ion chromatography , sugar , nuclear magnetic resonance spectroscopy , biochemistry , stereochemistry , bacteria , phosphorylation , biology , lactic acid , genetics , phosphatase
Glycopolymers of two types were isolated from the cell wall of Lactococcus lactis BIM B-1024 by stepwise extraction with cold and hot 10% CCl 3 CO 2 H and separated by anion-exchange gel chromatography. The following structures of the glycopolymers were established by sugar analysis, dephosphorylation with 48% HF, 1D and 2D NMR spectroscopy, and ESI-MS: β-D-Galp-(1→4)-β-D-Glcp-(1→6)┐ →4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp-(1→ PSI β-D-Glcp-(1→3)┐ →60-α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→5)-Rib-ol-1-P-(O→ PSII β-D-Glcp-(1→4)┘ Polysaccharides with the same or similar structures to PSI have been found earlier in various Lactobacillus species, whereas, to our knowledge, the structure of PSII is new.