Open AccessBIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF SOME ORGANS AND TISSUES OF HYBRID TILAPIA (OREOCHROMIS SPP.) FOR GROWING WITH USE OF PREPARATION ES-2
Author(s) -
Svetlana Kotelnikova,
Andrey Vyacheslavovich Kotelnikov,
Alexander Nickolaevich Nevalennyy,
S.V. Ponomarev,
Yulia Viktorovna Fedorovykh
Publication year - 2018
Publication title -
vestnik astrahanskogo gosudarstvennogo tehničeskogo universiteta. seriâ: rybnoe hozâjstvo
Language(s) - English
Resource type - Journals
eISSN - 2309-978X
pISSN - 2073-5529
DOI - 10.24143/2073-5529-2018-3-113-117
Subject(s) - gill , lipid peroxidation , oreochromis , tilapia , chemistry , antioxidant , biology , catalase , biochemistry , nile tilapia , hepatocyte , food science , fish <actinopterygii> , fishery , in vitro
The article studies the effect of addition into the feed of Sapropel extract (ES-2 preparation) on the intensity of lipid peroxidation in the liver and gills of hybrid tilapia ( Oreochromis spp. ), as well as on the morphofunctional state of its liver. Sapropel extract caused a decrease in the content of TBA-reactants in the tissues of tilapia liver by 17% compared to the control group. In gills the bioadditive resulted in the increased content of peroxide products by 24%. The introduction of ES-2 in fish feed resulted in reduction of spontaneous and ascorbate-dependent lipid peroxidation rate in the liver by 18%. In the gills of fish, under the influence of Sapropel, the rate of spontaneous lipid peroxidation increased by 27%, the rate of ascorbate-dependent lipid peroxidation - by 23%. The change in the intensity of peroxide processes under the influence of the fodder additive in fish organs is tissue-specific: antioxidant effect was recorded in the liver, prooxidant effect was observed in the gills. The introduction of the Sapropel extract does not lead to a change in the volume of liver nuclei in the test groups of tilapia, while the average cell volume in the experimental group was 37% lower than in the control group. The decrease in cell volume led to the increase in the nuclear-cytoplasmic ratio by 1.9 times in the experimental group compared to the control group. Hepatocyte cytoplasm volume decrease and nuclear-cytoplasmic ratio increase due to addition of ES-2 preparation into productive feed of hybrid tilapia would indicate a rise of functional activity of liver cells.