Open AccessThe activity of extracts chara vulgaris against promastigotes of leishmania tropica.
Author(s) -
Ban Hussein Ali,
Thaer A. Saleh,
Mohammed M. F. Al-Halbosiy
Publication year - 2018
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2018.12.2.540
Subject(s) - leishmania tropica , biology , leishmania major , leishmania , chlorella vulgaris , algae , traditional medicine , incubation , microbiology and biotechnology , bioassay , leishmaniasis , in vitro , bromide , botany , chemistry , cutaneous leishmaniasis , parasite hosting , biochemistry , medicine , immunology , ecology , world wide web , computer science , organic chemistry
Leishmaniasis is a widespread parasitic disease caused by Leishmania parasite, this disease considers as a major health problem worldwide. The available therapy is unsatisfactory expensive with a cytotoxic side effects. Studies of marine algae as a source of pharmacological active compounds have increased worldwide. This study was aimed to investigate the effect of a type of green algae (Chara vulgaris) on promastigotes of L. tropica, by using. various concentrations (500, 250, 125, 62.5, 31.25, 15.6 µg/mL) in vitro by MTT assay [3-(4.5-dimethylthiazol-2-yl)- 2.5-diphenyl tetrazolium bromide)], to investigate its effect on the proliferation of promastigotes, by three incubation periods (24, 48, 72 hr.) The results showed a significant (p< 0.05) decrease in survived of promastigotes in treatment groups with concentrations that ranged between 15 to 500 μg/ ml. This study revealed a major growth inhibition effect of the organic extract of C. vulgaris against L. tropica promastigotes, and the extract of ethyl acetate showed potential activity is better than the aqueous extract.