Open AccessEffects of Hair dyes on Sequence Analysis of Hair Mitochondrial DNA Hypervariable Region 1
Author(s) -
Halah Al-summarraie,
Mohammed Mahdi Al-Zubaidi,
Dhuha Salim نعمة,
Sura Nabeel Hameed,
Thooalnoon Younes Saleh,
Haider Al-rubai,
Dheaa Shamikh Zageer,
Ala Bader
Publication year - 2017
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2017.11.1.505
Subject(s) - mitochondrial dna , dna , hypervariable region , hair analysis , hair follicle , hair shaft , dna extraction , biology , nuclear dna , polymerase chain reaction , body hair , microbiology and biotechnology , chromatography , genetics , chemistry , anatomy , pathology , gene , medicine , alternative medicine
Hair can be a valuable source of DNA especially in forensic casework and for noninvasive studies of human, when blood samples not available. This study emphasizes the impact of hair dyes on DNA sequence analysis. Samples collected from forty Iraqi families; each sample was divided in to two parts hair follicles and hair shaft. DNA extracted by using two different techniques, Phenol-chloroform (organic) method and prepFiler forensic DNA extraction kit. After quantification of DNA by real time PCR to confirm the exact DNA yield, Mitochondrial DNA (MtDNA) hypervariable region 1 successfully amplified from (50% of samples include hair follicle and 20% samples include shaft only), which all extracted by organic method. Whereas by using prepFiler kit the ratio of amplification success reach to 95% of samples included hair follicles, but there wais no DNA outcome from hair shaft by using this kit. Our results demonstrate that treated hair by dyed or henna had a significant influence on the sequence analysis results. Organic method was an appropriate method for extraction DNA from hair shaft, since this method used for extracting the old and degraded samples. While prepFiler DNA extraction kit was more convenient for isolation DNA from, hair samples included follicles only with excellent result.