Open AccessCytarabine Induced Reproductive Histopathological Changes in Albino Male Mice
Author(s) -
Niran A. Ibrahim,
Hanady S. Al-Shmgani,
Ruqaya Mohammed Ibrahim
Publication year - 2017
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2017.11.1.489
Subject(s) - spermatogenesis , cytarabine , testosterone (patch) , seminiferous tubule , germinal epithelium , andrology , biology , saline , medicine , sperm , toxicity , endocrinology , hormone , leukemia , sertoli cell
Cytarabine (Ara-C) is widely used as an effective chemotherapeutic agent, essentially in the treatment of acute myeloid leukemia. Albino male mice (Mus musculus) are used in this study to determine in vivo toxicity of the drug on spermatogenesis and sperm abnormality. Mice are divided into two groups; the experimental group received a dose of 0.33 mg/kg/day Ara-C for seven days, while the control group received normal saline for 7 days. Results indicated a significant increase in head abnormalities percentage (p≤ 0.05) in comparison to control group. Testosterone hormone concentration was significantly decreased (1.14 ± 0.12) ng/ml in treatment group in comparison with control group (2.41± 0.22) ng/ml. Histopathological changes revealed detectable spermatogenesis degeneration. Treatment caused spermatocytes disruption, presence of necrotic cells and appearance of spermiophages with slought cells in several seminiferous tubules. Leydig cells morphology are mostly intact; however, interstitial area appeared hypercellular. It is concluded that Ara-c exposure highly destructive to seminiferous tubule epithelium, which may lead in depressed mice fertility.