Open AccessComparative Study of Expansion and Proliferation of Adult Mice Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue
Author(s) -
Mahfoodha A. Umran,
Marwa Salman
Publication year - 2016
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2016.10.2.481
Subject(s) - mesenchymal stem cell , adipose tissue , bone marrow , cd34 , stem cell transplantation for articular cartilage repair , stem cell , clinical uses of mesenchymal stem cells , regenerative medicine , immune system , tissue engineering , biology , microbiology and biotechnology , phenotype , adult stem cell , immunology , endothelial stem cell , in vitro , endocrinology , genetics , biochemistry , gene
The importance of Mesenchymal stem cells (MSCs) represents a favorable tool for new clinical concepts in supporting tissue engineering and cellular therapy. Bone marrow (BM) was considered important source contain mesenchymal stem cells .Another promising source of MSCs is adipose tissue (AT). MSCs derived from these sources compared regarding morphology, the success rate of isolating MSCs, expansion potential by rate of colony forming and immune phenotype. The obtained results from this study showed no obvious considerable differences concerning the morphology and immune phenotype of the MSCs derived from these sources were obvious. Differences observed concerning to the success rate of isolating MSCs, which was approximately more than 90% for BM, while it reached about 70% for AT after seven days of culturing, as well as the rate of colony forming was lower in AT cells in comparison to that obtained in BM at the same period. However, AT-MSCs could be required longest time to complete monolayer confluence, whereas BM-MSCs had the shortest proliferation period. Cells from both sources determined according to immunohistochemistry by CD105+ and CD34.¯ Conclusions revealed that MSCs can easily and successfully obtained from bone marrow and adipose tissues, and both tissues appears suitable sources of stem cells for potential use in regenerative medicine, repairing damaged tissue nevertheless the BM-MSCs more effectual in expansion and proliferation.