Open AccessPurification and Identification of Flavonoids Extracted from Loranthus Eurpaeus Fruits
Author(s) -
Eslah S. Rajab,
Khlood W. Abood,
Zaynab Y. Muhammed Hassn
Publication year - 2016
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2016.10.1.448
Subject(s) - maceration (sewage) , chemistry , chromatography , high performance liquid chromatography , ethyl acetate , thin layer chromatography , flavonoid , ethanol , methanol , organic chemistry , antioxidant , materials science , composite material
Loranthus eurpaeusis an important medicinal plant, which contains a lot of bioactive compounds. The dried plantfruits were extracted overnight with 80% methanol by maceration using shaker incubator 25c◦. Chemicaldetection of crud plant extracts was performed. The total flavonoids was isolated from the extract using reflux,and subjected to thin layer chromatography (TLC) using different mobile systems. The purified material wasaugmented by using high performance liquid chromatography (HPLC). The aglycon moiety was extracted byethyl acetate, and then evaporated to dryness. The dried residue then redissolved in 50% ethanol. Purificationprocedures of flavonoid were fully described in this study. The aims of this study was to detect the activecompounds present in L. eurpaeus methanolic extract then quantitative and qualitative estimation of the totalflavonoids isolated from the plant fruits.