Open AccessGenetic relation between three morphological states of N2-fixing Cyanobacteria by using PCR technique
Author(s) -
Rawaa D. Hamoshi Hamoshi,
Hammed S. Khamees Khamees,
Bassema A. Abdulla Abdulla
Publication year - 2015
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2015.9.1.403
Subject(s) - cyanobacteria , rapd , oscillatoria , biology , anabaena , botany , microcystis , polymerase chain reaction , microbiology and biotechnology , genetics , genetic diversity , bacteria , gene , population , demography , sociology
Three nitrogen fixing Cyanobacteria purified isolates were selected which express the threemorphological states, first Oscillatoria chlorina as an example of filamentous non HeterocystsCyanobacteria and second Microcystis rubusta as an example of Unicellular Cyanobactera andthird Anabaena spiroides as an example of filamentous Cyanobacteria containing a Hetrocysis,these isolate were cultured on ASM-1 free nitrogen medium using light tension of lux (2500) andtemperature of 25°C. Total DNA were isolated and found that its concentration varied between(1.3 to 1.8) the concentration was fixed in order to obtain that each 100 l contains 50 ng/l.Primers for RAPD-PCR were used to detect the genetic variation between the three genera ofCyanobacteria understudy and in order to diagnose the genetic relationship and to detect thegenetic dimension between these isolates in order to use these results to found the geneticfingerprint. Eleven primers were used in RAPD-PCR reaction and 10 of them were positive. TheOPC-05 gave the high number of bands (20 bands) while OPA-18 and OPJ-13 gave the lowest (9bands).The genetic dimension between the three isolates were compatible with the morphological,microscopical and physiological results obtained as we found that Microcyctis rubusta andAnabaena spiroides were closely related and Oscillatoria chlorine was far genetically.