Open AccessGenotyping of High-risk Human Papilloma virus (HPV) among Iraqi women in Baghdad by Multiplex PCR
Author(s) -
Ashna Jamal Faik,
Mudhafar Q. Saber Saber,
Wisam J. Mohammed Mohammed,
Bashar Z. Ibraheem Ibraheem,
Kawther R. Lateef Lateef,
Areeg S. Hassen Areeg S. Hassen
Publication year - 2015
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2015.9.1.402
Subject(s) - genotyping , human papilloma virus , cervical cancer , medicine , multiplex polymerase chain reaction , hpv infection , gynecology , genotype , population , typing , cytology , virology , polymerase chain reaction , cancer , biology , pathology , gene , genetics , environmental health
Human papilloma virus (HPV) infection is a causative factor for cervical cancer. Early detectionrisk HPV types might help to identify women at high risk of cervical cancer. The aim of of highHPV infection in population of rgir hgih determine the occurrence of the present study was toIraqi women in Baghdad by using Multiplex PCR determine the percentage and genotyping ofHuman Papilloma Virus and to put the best prevention and control program in Iraqi women.Study started at January 2009 to March 2010, cervical samples were collected from 856 womenaged 16–70. HPV DNA amplification was performed using HPV High Risk Typing PCR Kit testfor qualitative detection and genotyping of HPV types 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66 inHPV was detected in 106 ( 12,38% ) of the study population, with a range of the cervical swabs.16-70 years age groups. Results showed that the overall HPV prevalence twelve genotypes wereidentified, including HPV-33 (18.60%), HPV-35 (18.60%), HPV-56 (18.60%) ,HPV-39(10.85%),HPV-52 (10.08%), HPV-18 (7.75%), HPV-16 (4.65%), HPV-59 (4.65%), HPV-58(2.32%), HPV-31(1.55%), HPV-45(1.55% ) and HPV-66( 0.77%). Of 856, 218 women was also tested by pap smearith normal cytology was 198 ( 90.83%), 24(12.12%) of them were HPV positive, those with w,abnormal cytology was 20 (9.17 %), 5( 25%)of them was HPV positive. In this study unlike otherepidemiological studies, HPV33,35,56 was the most frequent type (55.8%) in Baghdad, followedby HPV39, HPV52, HPV18, HPV16.