Open AccessEffect of Naphthalene acetic acid and date palm pollen extraction on gene expression on fruit of Hillawi date palm cultivar during growth and ripening
Author(s) -
Aqeel Hadi Abdulwahed Abdulwahed
Publication year - 2015
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2015.9.1.400
Subject(s) - ripening , cultivar , biology , palm , pollination , pollen , phoenix dactylifera , horticulture , botany , prunus , physics , quantum mechanics
This study was carried out on fruit of Hillawi date palm cultivar which pollinated by Khukri Adi andGhunami Akhder cultivars to study the effect of NAA 25 ppm and pollen extraction of Khukri Adi andGhunami Akhder 2g/L which were sprayed at 30 and 45 day after pollination on gene expression bystudying the changes in protein pattern during fruit development and ripening by gel electrophoresesmethod with sodium dodecyle sulfate (SDS-PAGE). The result showed there were many protein bandwhich were different in molecular weight and numbers on the gel with changes on fruit growth stages andtreatments, These bands of protein were similar in early stage of growth but different in the period of13,15 weeks after pollination, this difference may be correlated with ripening date. Our results showedthat we could consider the protein pattern as gene expression markers for fruit and linking them with adifferent growth and ripening stage and explanation of metaxenia phenomenon.