Open AccessThe mutagenicity and anti-mutagenicity effect of aqueous leaves extract of Vinca rosea by bacterial system (Part one)
Author(s) -
Ali Hafedh Abbas,
Likaa Hasoun Sagban Sagban,
Hind Hussein Obaid,
Ishrak Abdul Ameer Abdul Ameer,
Rasha Hussein Kubba Kubba,
Shaymaa Adnan Ez-Aldeen Ez-Aldeen
Publication year - 2015
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2015.9.1.399
Subject(s) - mutagen , vinca , chemistry , aqueous solution , streptomycin , biology , antibiotics , pharmacology , biochemistry , dna , organic chemistry
This study was carried out in order to determine the toxic, mutagenic and anti-mutagenic effectsfor aqueous extract of Vinca rosea dried leaves against the mutagenic effect of Methotrexate(MTX) as a chemical mutagen and Ultra Violet rays (UV) as a physical mutagen. The effect wasstudied in a bacterial system (G-system). The used system consisted of three wild isolatesG3Bacillus spp., G12Arthrobacter spp. and G27Brevibacterium spp., The study depended onrecording survival fraction (Sx) as an indicator for the induction of Streptomycin and Rifampic inresistance mutants as a genetic marker. Aqueous extract was prepared from dried leaves, gradualconcentrations of plant aqueous extract was used to choose the suitable concentration which isremembered the negative control, the optimum concentration of aqueous extract was 200 µl/mlcomparing with the negative control. The interactions included three types of treatments (premutagen, with-mutagen and post-mutagen) in order to determine the mechanisms of this plantextracts in preventing or reducing the genotoxic effect of mutagen (MTX and UV).The resultsshowed that the interaction effect between the optimum concentration of aqueous extract and themutagen on survival fraction (Sx) increase the survival fraction value of the of G-system isolates toreach normal value compare with positive control (MTX), the results of the interaction betweenoptimum concentration for extracts and the treatment with mutagen to induce resistance mutantfor streptomycin and rifampicin found that the MTX had no effect to induce resistance mutantfor these two antibiotics, for all types of treatment (pre-MTX, with-MTX, and post-MTX), theaqueous extract suppress or repair mutant and give 100% protection for bacterial cells, while theUV had some effects to induce resistance mutant for these two antibiotics for pre-UV and withinUV treatments and the aqueous extract showed suppressing or repairing mutant partially withpost-UV treatment and gave 100% protection for bacterial cells.