Open AccessIdentification of diosgenin isolated from genetically engineered tissues cultures of Trigonella foenum-graecum L.
Author(s) -
M. M. Al-Mahdawe,
¬ M. K. Al-Mallah,
A. O. Al-Attrakchii
Publication year - 2014
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2014.8.2.318
Subject(s) - diosgenin , trigonella , agrobacterium , callus , high performance liquid chromatography , chemistry , botany , biology , chromatography , transformation (genetics) , biochemistry , gene , organic chemistry
Thediosgenin compound was separated, one of the secondary metabolic product in Trigonella foenum-graecum L. plants. Hairy roots cultures transformed by Agrobacterium rhizogenes R1601, and their spontaneously formed callus contained "diosgenin". High performance liquid chromatography HPLC measurements proved the presence of diosgenin in these cultures that approach 158.3 and 156.5% respectively. It present in leaves of plants produced from seeds at 161.8%. Generally, these data pointed out that transformed tissues are often preferred as a continuous source of diosgenin and perhaps other secondary metabolites.