Open AccessInduction of murine malignant lymphoma by 1-(2-chloro-ethyl)-3-cyclo-hexyl-1-nitrosourea, In vitro partial characterization
Author(s) -
Ahmed Majeed Al-Shammari
Publication year - 2013
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2013.7.1.247
Subject(s) - nitrosourea , lymphoma , in vitro , cell culture , cancer research , carcinogen , malignant lymphoma , biology , chemistry , immunology , chemotherapy , biochemistry , genetics
From 20 mice administered ethyl nitrosourea for over 5 months, 2 mice showed lung lymphoma, one of them is a murine malignant lymphoma which cultured and the cell line was newly established as short term culture. The cells were round in shape and had a tendency to make groups of floating clusters. Later these cells developed to proliferate as adherent cell culture. Our study showed that ethyl nitrosourea is selective carcinogen to induce lymphoma and lymphoma cultured cells is very useful for lymphoma studies.