Open AccessUsing PCR Technician in Prenatal Diagnosis of Fetal Gender
Author(s) -
Abdulrahman A. Oleiwi
Publication year - 2010
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2010.4.2.130
Subject(s) - fetus , ethidium bromide , gestation , agarose gel electrophoresis , prenatal diagnosis , andrology , pregnancy , obstetrics , medicine , cell free fetal dna , biology , dna , genetics
whole blood samples were obtained from 30 pregnant women at 15 –24 weeks of gestation. DNA was extracted from each plasma or serum sample. To detect the Y-chromosome specific marker DYS14 in the maternal blood, (Polymerase Chain Reaction) PCR were carried out for each DNA extract. The PCR products were analyzed by 1.5% agarose gel electrophoresis and ethidium bromide staining. The results compared with fetal gender after delivery. The result of delivery revealed that 13 pregnant women had a male fetus and the remaining 17 pregnant women had a female fetus and DYS14 was detected in all plasma and serum samples obtained from pregnant women and revealed that 13 pregnant women had a male fetus and the remaining 17 pregnant women had a female fetus. The PCR sensitivity for detecting the gender of fetus from maternal whole blood at 15–24 weeks of gestation was 100% in both plasma and serum, DYS14 was not detected in the DNA from any of the 17 pregnant women carrying a female fetus. The results showed that PCR analysis of maternal plasma and serum can be used to diagnose fetal gender.