Open AccessIdentifcation of some local wheat varieties by high performance liquid chromatography technique
Author(s) -
Makarim Ali Mousa,
Farook Fathel. Al nouri,
Sabri Ch. Abood
Publication year - 2010
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2010.4.2.112
Subject(s) - gliadin , high performance liquid chromatography , cultivar , chromatography , chemistry , biology , food science , gluten , horticulture
Gliadin protein of three soft wheat cultivars namely Abu-Ghraib, IPA 99 and AL-Littefia was extracted and separation by high performance liquid chromatography HPLC. Result showed that there were significant differences between the gliadin percentage ratio with the order of AL-Littefia 34.02% , IPA99 30.20% and Abu-Ghraib 31.00% . Also the result of HPLC analysis revealed that the gliadin of the test cultivars has 3 distinguish patterns of separation. Thus it appears that HPLC provides efficient tool for identification of wheat cultivars. Abu-Ghraib gliadin showed 6 peaks while IPA99 and AL-Littefia showed 15 and10 peaks respectively.