Effects of Antrodia Camphorata Supplementation on Anti-fatigue Effects in Rats

LANGUAGE NOTE | Document text in English; abstract also in Chinese. This study investigated the effects of antrodia camphorata (AC) supplementation on anti-fatigue effects after a single bout of exhaustive exercise in rats. Thirty-two rats were randomly divided into four groups: control (C, n=8); C with antrodia camphorata supplementation (A, n=8); exhaustive exercise (E, n=8); exhaustive exercise with AC supplementation (AE, n=8). Rats in groups A and AE received a 0.2 c.c. (kg/wt/day) AC supplementation for two weeks. Rats in groups E and AE performed an exhaustive running test on a treadmill at a final speed of 30m/min, 10% grade, at approximately 70-75% VO2max. Blood samples of each rat were collected. The concentrations of glucose, lactate dehydrogenase (LDH), uric acid, creatinine, and creatine kinase (CK) in blood were examined. The results showed a significant increase in exhaustive exercise time of AE group (78.79±9.09 min) compared with E group (67.77±9.22 min). The activities of CK, LDH, and blood creatinine concentration significantly increased in exercised rats (1227.40±553.24 U/I, 2108.32±513.99 U/I, 0.76±0.19 mg/ dl) compared with control group rats (358.98±125.53 U/I, 956.89±597.13 U/I, 0.5±0.15 mg/dl) and AE group rats (774.13±203.38 U/I, 1300.93±513.99 U/I, 0.5±0.05 mg/dl). The glucose in the plasma significantly decreased in E group rats (133.88±61.84 mg/dl) compared with C group rats (209.63±69.13 mg/dl) and AE group rats(188.88±60.32 mg/dl). The results indicate that AC supplementation could decrease CK, LDH activities. It may help for anti-fatigue effects. 本研究的目的在探討樟芝補充劑對老鼠經單次衰竭運動後對抗疲勞作用的影響三十二隻老鼠隨機分配為以下四組控制組(C, n=8) 、樟芝組(A, n=8補充樟芝沒有運動)、運動組(E, n=8)、樟芝運動組(AE, n=8補充樟芝有運動)補充樟芝組(A & AE)的老鼠每天補充每公斤體重0.2毫升的樟芝補充劑持續兩週衰竭運動組(E & AE)的老鼠需在鼠用跑步機上進行衰竭運動運動強度約70~75%最大攝氧量最終跑步機速度為30公尺/分鐘坡度為10%。收集老鼠血液樣本測量血糖、乳酸去氫酶、尿酸、肌酸酐、肌酸激酶。研究結果顯示AE組運動衰竭時間(78.79±9.09 min)顯著比E組(67.77±9.22 min)長。運動組(E)的肌酸激酶活性、乳酸去氫酶活性、肌酸酐(1227.40±553.24 U/I, 2108.32±513.99 U/I, 0.76±0.19 mg/dl)顯著比控制組(C)(358.98± 125.53 U/I, 956.89±597.13 U/I, 0.5±0.15 mg/dl)及樟芝運動組(AE)高(774.13±203.38 U/I, 1300.93±513.99 U/ I, 0.5±0.05 mg/dl)。運動組(E)血糖濃度(133.88±61.84 mg/dl)顯著比控制組(209.63±69.13 mg/dl)及樟芝運動組(188.88±60.32 mg/dl)低。研究結果表示樟芝補充能夠降低肌酸激酶活性及乳酸去氫酶活性有助於抗疲勞的效用。