Open AccessGenetic diversity within the 18S rRNA and actin locus of Cryptosporidium scrofarum (Apicomplexa: Cryptosporidiidae) infecting domestic pigs (Sus scrofa domesticus) of India
Author(s) -
Dipika Sharma,
Nirbhay Kumar Singh,
Harkirat Singh,
S. S. Rath,
Damer P. Blake
Publication year - 2021
Publication title -
veterinarski arhiv
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.233
H-Index - 29
eISSN - 1331-8055
pISSN - 0372-5480
DOI - 10.24099/vet.arhiv.0674
Subject(s) - biology , cryptosporidium , 18s ribosomal rna , genotype , amplicon , genetic diversity , locus (genetics) , phylogenetic tree , apicomplexa , genetics , ribosomal rna , domestic pig , gene , polymerase chain reaction , microbiology and biotechnology , population , feces , demography , immunology , sociology , malaria , plasmodium falciparum , forestry , geography
The genetic diversity was studied of Cryptosporidium scrofarum (syn Cryptosporidium pig genotype II) of domestic pigs (Sus scrofa domesticus) from Punjab, India. Nested PCR amplification targeting the 18S rRNA and actin gene loci from Cryptosporidium positive samples was carried out, and the amplicons were sequenced. Phylogenetic comparison of a partial 18S rRNA gene revealed that they were genetically most similar to C. scrofarum isolated from other parts of the world. However, comparison of sequences representing a fragment of the genomic actin locus identified a new genotype conserved within the isolates sampled from India but distinct from other published sequences, suggesting the presence of a different Indian genotype.